Delipidated Fetal Bovine Serum
Triple 0.1 um Filtered
10,000MW Cutoff Filter Further filtered
USDA Safety Tested and Approved
Delipidated Fetal Bovine Serum
NZF-DE-050 50mL
NZF-DE-100 100mL
NZF-DE-300 6 X 50mL
NZF-DE-500 500mL
The high-quality fetal bovine serum collected from New Zealand will be further processed to obtain this series of products. Fetal Bovine Serum (FBS), Delipidated is used for applications, where a reduced lipid concentration in FBS is required, such as lipid metabolism studies or arteriosclerosis research.
Fetal Bovine Serum (FBS), Delipidated is produced using the fumed silica precipitation method. When fumed silica is added to serum, non-polar substances, such as lipids and steroids, adsorb to the surface of the silica. The solution is then centrifuged and the pellet of fumed silica with adsorbed lipids is removed. The serum is then sterile-filtered. The acceptance criterion for the treatment is a level of cholesterol lower than 10 mg/100 ml. Lipids and other non-polar substances are reduced in comparison to non-treated serum.
Intended Use
For Further Manufacturing or Research Use Only. Not for Diagnostic or Therapeutic Use.
Use Instructions
For use in cell culture media, the supplementation ranges from 5% to 20%. The optimum concentration concentration should be determined by the ultimate user.
Heat Inactivation
Serum may be heat inactivated at 56°C for 30 minutes.
Storage and Stability
Store at -10°C to-40°C. Product is stable for 5 years from the date of manufacture if kept at frozen temperature. We suggest that the product should not be subjected to multiple freeze-thaw cycles, should be made into aliquots. Turbidity or flocculent material may be observed upon thawing or prolonged storage at refrigerated temperatures. This is normal in most serum products and not detrimental to its performance.
Finished Product Specifications
Endotoxin ≤ 10 EU / ml
Hemoglobin (spectrophotometric) ≤10 mg / dl
Sterility Testing (Current USP)
Bacteria Not Detected
Fungi Not Detected
Virus Testing (9CFR 113.53) Not Detected
Bovine Viral Diarrhea Virus Not Detected
Cytopathogenic Agents (IBR) Not Detected
Hemadsorbing Agents (PI3) Not Detected
Mycoplasma Not Detected