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Dulbecco's Modified Eagle's Medium - Low GlucoseLiquid media-Concentration (mg/L)

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12.

Amino Acids

Glycine	30
L-Arginine hydrochloride	84
L-Cystine 2HCl	63
L-Glutamine	584
L-Histidine hydrochloride-H2O	42
L-Isoleucine	105
L-Leucine	105
L-Lysine hydrochloride	146
L-Methionine	30
L-Phenylalanine	66
L-Serine	42
L-Threonine	95
L-Tryptophan	16
L-Tyrosine disodium salt dihydrate	104
L-Valine	94

Vitamins

Choline chloride	4
D-Calcium pantothenate	4
Folic Acid	4
Niacinamide	4
Pyridoxine hydrochloride	4
Riboflavin	0.4
Thiamine hydrochloride	4
i-Inositol	7.2

Inorganic Salts

Calcium Chloride (CaCl2) (anhyd.)	200
Ferric Nitrate (Fe(NO3)3"9H2O)	0.1
Magnesium Sulfate (MgSO4) (anhyd.)	97.67
Potassium Chloride (KCl)	400
Sodium Bicarbonate (NaHCO3)	3700
Sodium Chloride (NaCl)	6400
Sodium Phosphate monobasic (NaH2PO4-H2O)	125