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Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12 DMEM/F12 (1:1) Concentration (mg/L)

DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts.

Amino Acids

Glycine	18.75	18.75	18.75	18.75
L-Alanine	4.45	4.45	4.45	4.45
L-Arginine hydrochloride	147.5	147.5	147.5	147.5
L-Asparagine-H2O	7.5	7.5	7.5	7.5
L-Aspartic acid	6.65	6.65	6.65	6.65
L-Cysteine hydrochloride-H2O	17.56	17.56	17.56	17.56
L-Cystine 2HCl	31.29	31.29	31.29	31.29
L-Glutamic Acid	7.35	7.35	7.35	7.35
L-Glutamine	365	0	365	365
L-Histidine hydrochloride-H2O	31.48	31.48	31.48	31.48
L-Isoleucine	54.47	54.47	54.47	54.47
L-Leucine	59.05	59.05	59.05	59.05
L-Lysine hydrochloride	91.26	91.26	91.26	91.26
L-Methionine	17.24	17.24	17.24	17.24
L-Phenylalanine	35.48	35.48	35.48	35.48
L-Proline	17.25	17.25	17.25	17.25
L-Serine	26.25	26.25	26.25	26.25
L-Threonine	53.45	53.45	53.45	53.45
L-Tryptophan	9.02	9.02	9.02	9.02
L-Tyrosine disodium salt dihydrate	55.79	55.79	55.79	55.79
L-Valine	52.85	52.85	52.85	52.85

Vitamins

Biotin	0.0036	0.0036	0.0036	0.0036
Choline chloride	8.98	8.98	8.98	8.98
D-Calcium pantothenate	2.24	2.24	2.24	2.24
Folic Acid	2.65	2.65	2.65	2.65
Niacinamide	2.02	2.02	2.02	2.02
Pyridoxine hydrochloride	0	2	0	0
Riboflavin	0.22	0.22	0.22	0.22
Thiamine hydrochloride	2.17	2.17	2.17	2.17
Vitamin B12	0.68	0.68	0.68	0.68
i-Inositol	12.6	12.6	12.6	12.6

Inorganic Salts

Calcium Chloride (CaCl2) (anhyd.)	116.6	116.6	116.6	116.6
Cupric sulfate (CuSO4-5H2O)	0.0013	0.0013	0.0013	0.0013
Ferric Nitrate (Fe(NO3)3"9H2O)	0.05	0.05	0.05	0.05
Ferrous Sulfate (FeSO4 7H2O)	0.417	0.417	0.417	0.417
Magnesium Chloride (anhydrous)	28.61	28.61	28.61	28.61
Magnesium Sulfate (MgSO4) (anhyd.)	48.84	48.84	48.84	48.84
Potassium Chloride (KCl)	311.8	311.8	311.8	311.8
Sodium Bicarbonate (NaHCO3)	1200	1200	1200	1200
Sodium Chloride (NaCl)	6999.5	6999.5	6999.5	6999.5
Sodium Phosphate dibasic (Na2HPO4) anhydrous	71.02	71.02	71.02	71.02
Sodium Phosphate monobasic (NaH2PO4-H2O)	62.5	62.5	62.5	62.5
Zinc sulfate (ZnSO4-7H2O)	0.43	0.43	0.43	0.43