On the way to the pertinent answer.
Iscove's Modified Dulbecco's Medium (IMDM) Liquid media-Concentration (mg/L)
Iscove's Modified Dulbecco's Medium (IMDM) is well suited for rapidly proliferating, high-density cell cultures, including Jurkat, COS-7, and macrophage cells.
Amino Acids
Glycine |
30 |
30 |
L-Alanine |
25 |
25 |
L-Arginine hydrochloride |
84 |
84 |
L-Asparagine (freebase) |
28 |
28 |
L-Aspartic acid |
30 |
30 |
L-Cystine 2HCl |
91.3 |
91.3 |
L-Glutamic Acid |
75 |
75 |
L-Glutamine |
584 |
0 |
L-Histidine |
42 |
42 |
L-Isoleucine |
105 |
105 |
L-Leucine |
105 |
105 |
L-Lysine hydrochloride |
146 |
146 |
L-Methionine |
30 |
30 |
L-Phenylalanine |
66 |
66 |
L-Proline |
40 |
40 |
L-Serine |
42 |
42 |
L-Threonine |
95 |
95 |
L-Tryptophan |
16 |
16 |
L-Tyrosine disodium salt |
104 |
104 |
L-Valine |
94 |
94 |
D-Biotin |
0.012 |
0.012 |
Choline chloride |
4 |
4 |
D-Calcium pantothenate |
4 |
4 |
Folic Acid |
4 |
4 |
Niacinamide |
4 |
4 |
Pyridoxal hydrochloride |
4 |
4 |
Riboflavin |
0.4 |
0.4 |
Thiamine hydrochloride |
4 |
4 |
Vitamin B12 |
0.013 |
0.013 |
i-Inositol |
7.2 |
7.2 |
Calcium Chloride (CaCl2) |
165 |
165 |
Magnesium Sulfate |
97.67 |
97.67 |
Potassium Chloride (KCl) |
330 |
330 |
Potassium Nitrate (KNO3) |
0.08 |
0.08 |
Sodium Chloride (NaCl) |
4505 |
4505 |
Sodium Phosphate |
125 |
125 |
Sodium Selenite |
0.015 |
0.015 |
D-Glucose (Dextrose) |
4500 |
4500 |
HEPES |
5958 |
5958 |
Phenol Red |
15.8 |
15.8 |
Sodium Pyruvate |
110 |
110 |
Sodium Bicarbonate (NaHCO3) |
3024 | 3024 |
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