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Leibovitz's L-15 Medium (liquid )Concentration (mg/L)
Leibovitz's L-15 Medium supports the growth of HEP-2 monkey kidney cells and primary explants of embryonic and adult human tissue. L-15 is buffered by phosphates and free base amino acids instead of sodium bicarbonate. This medium is designed for supporting cell growth in environments without CO2 equilibration.
Amino Acids
Glycine |
200 |
L-Alanine |
225 |
L-Arginine |
500 |
L-Asparagine |
250 |
L-Cysteine |
120 |
L-Glutamine |
300 |
L-Histidine |
250 |
L-Isoleucine |
125 |
L-Leucine |
125 |
L-Lysine |
75 |
L-Methionine |
75 |
L-Phenylalanine |
125 |
L-Serine |
200 |
L-Threonine |
300 |
L-Tryptophan |
20 |
L-Tyrosine |
300 |
L-Valine |
100 |
Choline chloride |
1 |
D-Calcium pantothenate |
1 |
Folic Acid |
1 |
Niacinamide |
1 |
Pyridoxine hydrochloride |
1 |
Riboflavin 5'-phosphate Na |
0.1 |
Thiamine monophosphate |
1.1 |
i-Inositol |
2 |
Calcium Chloride (CaCl2) (anhyd.) |
140 |
Magnesium Chloride (anhydrous) |
93.7 |
Magnesium Sulfate (MgSO4) (anhyd.) |
97.67 |
Potassium Chloride (KCl) |
400 |
Potassium Phosphate monobasic (KH2PO4) |
60 |
Sodium Chloride (NaCl) |
8000 |
Sodium Phosphate dibasic (Na2HPO4) anhydrous |
190 |
D+ Galactose |
900 |
Phenol Red |
10.6 |
Sodium Pyruvate |
550 |
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