Ham's F-10 Nutrient Mix Liquid media Concentration (mg/L)
Ham's F-10 Nutrient Mixture (F-10) was designed for serum-free growth of Chinese Hamster Ovary (CHO) cells. F-10 has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including COS-7, primary rat astrocytes, and rat prostate epithelial cells.
Amino Acids
|
Glycine |
7.5 |
7.5 |
|
L-Alanine |
8.92 |
8.92 |
|
L-Arginine hydrochloride |
211 |
211 |
|
L-Asparagine-H2O |
15 |
15 |
|
L-Aspartic acid |
13.2 |
13.2 |
|
L-Cysteine |
25 |
25 |
|
L-Glutamic Acid |
14.7 |
14.7 |
|
L-Glutamine |
146 |
146 |
|
L-Histidine hydrochloride-H2O |
23 |
23 |
|
L-Isoleucine |
2.6 |
2.6 |
|
L-Leucine |
13.1 |
13.1 |
|
L-Lysine hydrochloride |
29.3 |
29.3 |
|
L-Methionine |
4.49 |
4.49 |
|
L-Phenylalanine |
5 |
5 |
|
L-Proline |
11.5 |
11.5 |
|
L-Serine |
10.5 |
10.5 |
|
L-Threonine |
3.6 |
3.6 |
|
L-Tryptophan |
0.6 |
0.6 |
|
L-Tyrosine disodium salt dihydrate |
2.62 |
2.62 |
|
L-Valine |
3.5 |
3.5 |
Inorganic Salts
D-Biotin
0.02
0.02
Choline chloride
0.71
0.71
D-Calcium pantothenate
0.7
0.7
Folic Acid
1.32
1.32
Niacinamide
0.61
0.61
Pyridoxine hydrochloride
0.2
0.2
Riboflavin
0.39
0.39
Thiamine hydrochloride
1.01
1.01
Vitamin B12
1.36
1.36
Thymidine
0.73
0.73
Lipoic Acid
0.2
0.2
Hypoxanthine Na
5.4
5.4
i-Inositol
0.5
0.5
Other Components
Calcium Chloride (CaCl2) (anhyd.)
33.29
33.29
Cupric sulfate (CuSO4-5H2O)
0.0025
0.0025
Ferric sulfate (FeSO4-7H2O)
0.834
0.834
Magnesium Sulfate (MgSO4) (anhyd.)
74.64
74.64
Potassium Chloride (KCl)
285
285
Potassium Phosphate monobasic (KH2PO4)
83
83
Sodium Chloride (NaCl)
7400
7400
Sodium Phosphate dibasic (Na2HPO4) anhydrous
153.7
153.7
Zinc sulfate (ZnSO4-7H2O)
0.03
0.03
D-Glucose (Dextrose)
1100
1100
HEPES
0
5958
Phenol Red
1.2
1.2
Sodium Pyruvate
110
110
Sodium Bicarbonate (NaHCO3)
1200
1200
