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Medium 199（M199） Concentration (mg/L)

Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells. Medium 199 is widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse pancreatic epithelium and rat lens tissues.

Amino Acids

Glycine	50
L-Alanine	25
L-Arginine HCL	70
L-Aspartic acid	30
L-Cysteine hydrochloride-H2O	0.11
L-Cystine 2HCl	26
L-Glutamic Acid	75
L-Glutamine	100
L-Histidine hydrochloride-H2O	21.88
L-Hydroxyproline	10
L-Isoleucine	40
L-Leucine	60
L-Lysine hydrochloride	70
L-Methionine	15
L-Phenylalanine	25
L-Proline	40
L-Serine	25
L-Threonine	30
L-Tryptophan	10
L-Tyrosine disodium salt dihydrate	57.66
L-Valine	25

Vitamins

Ascorbic Acid	0.05
D-Biotin	0.01
Choline chloride	0.5
D-Calcium pantothenate	0.01
Folic Acid	0.01
Menadione (Vitamin K3)	0.01
Niacinamide	0.3
Niacin	0.3
Pyridoxal hydrochloride	0.025
Pyridoxine hydrochloride	0.025
Riboflavin	0.01
Thiamine hydrochloride	0.01
Vitamin A (acetate)	0.14
Vitamin D2 (Calciferol)	0.1
alpha Tocopherol phos. Na salt	0.01
i-Inositol	0.05

Inorganic Salts

Calcium Chloride (CaCl2) (anhyd.)	200
Ferric nitrate (Fe(NO3)-9H2O)	0.72
Magnesium Sulfate (MgSO4)	97.67
Potassium Chloride (KCl)	400
Sodium Chloride (NaCl)	6800
Sodium Phosphate monobasic (NaH2PO4-H2O)	140
Sodium Acetate	83

Other Components

Adenine sulfate	10
ATP-2Na	1
AMP	0.2
2-deoxy-D-ribose	0.5
Guanine hydrochloride	0.3
Hypoxanthine Na	0.4
D-Glucose (Dextrose)	1000
D-Ribose	0.5
Thymine	0.3
Uracil	0.3
Xanthine-Na	0.34
Glutathione (reduced)	0.05
Phenol Red	20
Para-Aminobenzoic Acid	0.05
Cholesterol	0.2
Tween 80	20
Sodium Bicarbonate (NaHCO3)	2200