Human ESC/iPSC medium
Description
Human ESC/iPSC medium is designed for human induced pluripotent stem cells (iPSCs) and human embryonic stem cells (ESCs).It is the serum-free medium consisting of synthetic chemicals or purified recombinant proteins.Human ESC/iPSC medium supports feeder-free growth and maintenance of human iPSCs or human ESCs on extra-cellular matrix proteins (e.g., BD Matrigel™). Its performance is comparable to the market-leading mTeSR™1 in growth rate, characteristic morphology, pluripotent markers, normal karyotype, and differentiation to germ layers.
Intended Use
For Further Manufacturing or Research Use Only. Not for Diagnostic or Therapeutic Use.
Storage and Shelf Life:
|
Contents |
Size |
Storage |
Shelf Life |
|
Basal Medium |
450mL |
2 to 8°C, protect from light |
6 months |
|
10×Supplement |
50mL |
-20°C or below,protect from light |
6 months |
Additional key reagents required or suggested
Extracellular matrix proteins, such as Matrigel™ (BD, cat# 354277) or equivalent
Accutase (Millipore, SCR005) or Collagenase IV (Invitrogen, 17104) or equivalent
ROCK inhibitor or Thiazovivin or equivalent
Adaptation of growing feeder-free cells to Human ESC/iPSC medium
Human iPSCs or ESCs that have been grown in most commonly-used hiPSC/ESC media can be easily adapted into Human ESC/iPSC medium. One round of 1:1 dilution of serum-containing or serum-free medium with Human ESC/iPSC medium is generally sufficient. However, for certain media and cell lines, a longer and more gradual adaptation may be required.
